Action de l’acriflavine sur les levures
APPENDIX In this appendix media and you will buffers required for the new actions produced in the preceding parts of that it chapter try indexed. Media
Slonimski, P
BMM. step 1.5 grams malt pull and you can 20 grams agar during the step 1 L cornmeal extract. Cornmeal pull is taken from 250 g cornmeal incubated in the 10 L drinking water from the 60°C straight away. After that timing the latest supernatant are filtered because of several layers regarding cheesecloth, therefore the cornmeal is actually discarded. CM medium. 0.15% MzP04,0.05% KCI, 0.05% MgS04,1% D- glucose, 0.37% NHEI, 0.2% Pepton, 0.2% ekÅŸi happn yeast pull, step 1 m& ZnS04,l mg/L FeCb Buffers Denaturation bufler: 1.5 Yards NaCI, 0.5 Yards NaOH Hybridization boundary: 50% Formamide (stringent hybridization), 5 X SSPE, 0.5% salt dodecyl sulfate (SDS), 0.step one mg/mL fish cum DNA. (The latest stringency of hybridization ide). Mitochondria barrier: 0.05 Yards Tris/Cl, 0.01 M EDTA, 0.5 Yards sucrose,pH 8.3 Mitochondria rysis barrier: 1% SDS, 0.05 M EDTA, 0.02 Meters salt acetate, pH 5.0; autoclaved Neutralization boundary: dos Yards NaCI, 1 Yards Tris/Cl, pH 5.5 2OX SSC step 1 L includes 175.step three grams NaCl, 88.2 grams salt citrate, pH 7.0 (modified with 10 N NaOH) 20X SSPE step one L contains 174 grams NaCl, twenty seven.six grams NaH2P04X H20, seven.cuatro g EDTA, pH adjusted to eight.4 having 10 N NaCl TE: 10 mM Tris/CI, step 1 mM EDTA, pH 8.0 TES: 31 mM Tris/CI, 5 mM EDTA, 50 mM NaCI, pH 8.0 TESISDS: 31 rnM Tris/CI, 5 mM EDTA, fifty mM NaCI, 4% SDS, pH 8.0 TESICsCl: Create step 1.1 grams CsCl for each and every mL TES and to alter refraction directory to 1.3985
) : eight hundred mM sodium acetate, 800 mM Tns/Cl, forty mM EDTA, pH 8.step 3 adjusted that have acetic acid GTC/PME barrier: 5.5 Yards Guanidium isothiocyanate,0.5% sarcosyl, 25 mM sodium citrate, 0.1 Yards P-mercaptoethanol,pH 7.0 RNA CsCI: 5.seven Yards CsCl, 0.1 Yards EDTA, pH 7.4 Records 1. Lederberg, J. (1952). Telephone genes and you can hereditary symbiosis. Physwl Rev. . dos. Esser, K. (1982). Cryptogumes. University Force, Cambridge. step three. P., B. Ephrussi (1949). V. Ce systeme de- cytochromes des mutants ‘petite colonie’. Ann. Inst. Pusteur Purh 77 419. 4. Osiewacz, H. D., J. Hermanns, D. Marcou, Meters. Triffi, K. Esser (1989). Mitochondrial DNA rearrangements is actually correlated which have a postponed amplification of one’s cellular intron (plDNA) within the a long-resided mutant of Podospom unserinu. Mutut. Res. 279:9. 5 . Rogers, H. J., K. W. Dollars, C. M.Brasier (1987). Amitochondrial address to own doublestranded RNA inside the infected isolates of your fungi that triggers Dutch elm situation. Characteristics 129558. 6. Wesolowski, Meters., H. Fukuhara (1981). Linear mitochondrial desoxyribonucleic acid throughout the yeast Hunsenulu mrukii. Mol. Mobile Biol. 1:387. 7. Kovacs, L., J. Lazowska, P. P. Slonimski (1984). A fungus having linear particles of mitochondrial DNA. Mol. Gen. Genet. 197420. 8. Zimmer, Yards.,G. Luckemann, B. F. Lang, K. Wolf (1984). The mitochondrial genome from fission yeast Schizosuccharomycespombe. step three. Gene mapping in the filter systems EFI (CBS 356) and you may study regarding hybrids ranging from strains EFI and you will ade seven-50 h-. Mol. Gen. Genet. 196473. 9. Hintz, W. E., Meters. Mohan, J. B. Anderson, P. Good. Horgen (1985). The mitochondrial DNA out of Agaricus: heterogeneity from inside the Good. bitorquis and you will homogeneity into the An effective. brunnescens. Cur.Genet. 9:127. ten. Hermanns, J., H. D. Osiewacz (1994). About three mitochondrial unassigned discover training frames out-of Podosporu unserinu show traces out of a viral-style of RNA polymerase gene. Spunk Genet. . 11. Stahl, U., P. An effective. Lemke, P. Tudzynski, U. Kuck, K. Esser (1978). Evidence for plasmid eg DNA when you look at the a beneficial filamentousfungi, the fresh new ascomycete Podospora unserinu. MoL Gen. Genet. 162341. twelve. Stahl, U., U. Kuck, P. Tudzynski,K. Esser (1980). Characterization and cloning from plasmid such as for instance DNA of ascomycete Podosporu unserinu. Mol Gen. Genet. 178 369. thirteen. Cummings, D. J., L. Belcour, C. Grandchamps (1979). Mitochondria1DNA from Podosporu unserinu. 11. Characteristics regarding mutant DNA and you may multimeric rounded DNA regarding senescent societies. Mol. Gen. Genet. 171